Journal of Clinical Nutrition & Dietetics

Effect of New Healthy and Viable Food Supplement on the Inhibition of Apoptosis and Increase of Neural Stem Cell Proliferation and Acute Toxicity of the Studied Diet in Rats

Objective: The aim of this study was to evaluate the effect of a new healthy and viable food supplement on the inhibition of apoptosis and increase of neural stem cell proliferation and acute toxicity of the studied diet in rats.

Methods: New healthy and viable food supplement were synthesized by a green route. This organic biomaterial was named NBS. Colorimetric assay for measuring the activity of enzymes (MTT) was used to assay cell proliferation and Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) assay to inhibit neural stem cell apoptosis. Wistar rats were also used to assay acute cytotoxicity. The mice were divided into three groups for treatment with food supplements and one group with 5 mice as control. Treatment groups were treated with the new healthy and live food supplement at concentrations of 500, 1000 and 1500 mg/kg for 28 days.

Results: The results of this study showed that concentration of 800 μg/ml was considered as the best concentration. The results of anti-apoptotic activity of extract showed that using healthy food supplement caused significant decrease in apoptosis in the healthy and live food supplement group compared to the control group. The results of acute toxicity analysis showed no increase or decrease in biochemical, hematological and pathological parameters in the studied animals. No specific macroscopic or microscopic signs of toxicity were observedand none of the mice studied in this research were killed during the study period.

Conclusion: The results showed that the new healthy and viable food supplement increases life expectancy.

Author(s): Azam Bayat*, Aref Khalkhali and Ali Reza Mahjoub

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