An antioxidant is of great interest among researchers, scientists, nutritionists, and the public because of its ability to prevent oxidative damage, as indicated by various studies. This chapter mainly focuses on the free radicals and their types; antioxidants and their mode of action against free radicals; fruits, vegetables, and their byproducts as a source of antioxidants; and various analytical methods employed for assessing antioxidant activity. Antioxidants discussed in this chapter are ascorbic acid, vitamin E, carotenoids and polyphenols, and their mechanism of action. Different antioxidant activity assay techniques have been reported. Fruits and vegetables are abundant sources of these secondary metabolites. The waste generated during processing has many bioactive materials, which possibly be used in value-added by-products.
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